Chicken and Rabbit Antibodies against Porcine Pepsinogen A
نویسندگان
چکیده
منابع مشابه
Chicken and rabbit antibodies against porcine pepsinogen A.
Isolated porcine pepsinogen A was used for the preparation of polyclonal rabbit and polyclonal chicken anti-pepsinogen A antibodies. Immunochemical properties of both immunoglobulin fractions were compared. The rabbit anti-serum was further purified using immobilized porcine pepsinogen A on magnetic cellulose beads and the resulting anti-pepsinogen A fraction proved to be applicable for the sep...
متن کاملPurification and characterization of chicken pepsinogen and chicken pepsin.
Chicken pepsinogen was extracted from the forestomach of chicken and purified by the consecutive application of acetone precipitation, chromatography on diethylaminoethyl cellulose, and gel filtration on Sephadex G-100. The purified pepsinogen was homogeneous on disc electrophoresis and sedimented as monodisperse material. Chicken pepsin was obtained by the activation of the pure zymogen at pH ...
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Crotalus durissus terrificus (C.d.t.) (South American rattlesnake) venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2) and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutr...
متن کاملIntramolecular activation of porcine pepsinogen.
Conversion of pepsinogen to pepsin at acid pH involves an intramolecular reaction in which the unproteolyzed zymogen cleaves itself. This conclusion is based upon experiments in which pepsinogen, at low concentrations, was activated in the presence of substrate, hemoglobin. Under these conditions, the activation of pepsinogen is independent of pepsinogen concentration, and addition of pepsin do...
متن کاملSpecificity of Chicken (IgY) versus Rabbit (IgG) Antibodies Raised against Cholecystokinin Octapeptide (CCK-8).
Antibodies produced in chickens (egg yolk Antibody-IgY) and rabbits against CCK-8 TyrSE (a C-terminal extended CCK-version) were compared with respect to their specificity against several modified CCK-sequences by means of radioimmunoassay and spot blot assay. The content of neuronal CCK was determined by using both an "avian" and a "mammalian" RIA. The IC50 values obtained indicate differences...
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ژورنال
عنوان ژورنال: Prague Medical Report
سال: 2015
ISSN: 1214-6994,2336-2936
DOI: 10.14712/23362936.2015.41